genotyping of haemophilus influenzae type b strains and their incidence in the clinical samples isolated from iranian patients

نویسندگان

somayeh bagherzadeh-khodashahri m.sc. student, department of microbiology, kerman science and research branch, islamic azad university, kerman, iran.

seyeddavar siadat department of mycobacteriology & pulmonary research, pasteur institute of iran, tehran, iran.

mohammad rahbar department of microbiology, reference health laboratories research center, ministry of health and medi- cal education, tehran, iran.

meghdad abdollahpour-alitappeh department of immunology, pasteur institute of iran, tehran, iran.

چکیده

background and objective: haemophilus influenzae type b (hib) is divided into two distinct genotypes, type i and type ii, based on the structure of capsular polysaccharides. the capsulation locus of haemophilus influenzae type b consists of three functionally distinct regions, designated regions 1 to 3. region iii contains hcsa and hcsb genes; however, notable sequence variation in this region can be used to recognize different hib genotypes. the purpose of this study was to investigate the prevalence and genotype of the hib strains isolated from patients with invasive disease in iran. materials and methods: in the present study, 8 pairs of primers were used for identification and serotyping of encapsulated haemophilus influenzae strains, as well as confirmation of species identification. additionally, in order to identify the cap- sular genotypes of haemophilus influenzae type b (type i and ii), two additional primer pairs were used to amplify the hcsa gene. results: out of 50 isolates of h. influenzae , four were found to be type b. interestingly, among these 4 hib isolates, 2 strains belonged to the type-ii category. conclusion: our study shows that the prevalence of both hib types i and ii seems to be high in iran.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genotyping of Haemophilus influenzae type b strains and their incidence in the clinical samples isolated from Iranian patients.

BACKGROUND AND OBJECTIVE Haemophilus influenzae type b (Hib) is divided into two distinct genotypes, type I and type II, based on the structure of capsular polysaccharides. The capsulation locus of Haemophilus influenzae type b consists of three functionally distinct regions, designated regions 1 to 3. Region III contains hcsA and hcsB genes; however, notable sequence variation in this region c...

متن کامل

Immunogenicity and Efficacy of Different Haemophilus influenzae type b Vaccines

Haemophilus influenzae, a major cause of meningitis in young children leading to death and other neurological sequelae. The disease leaves 15 to 35% of the survivors with permanent disabilities, such as, mental retardation or deafness. Despite the availability of new and more powerful antibiotics children with Hib meningitis still suffer from high mortality or morbidity. The emergence of multir...

متن کامل

Incidence of Haemophilus influenzae type b meningitis in India.

BACKGROUND & OBJECTIVE Vaccine policy depends on locally relevant disease burden estimates. The incidence of Haemophilus influenzae type b (Hib) disease is not well characterized in the South Asian region, home to 30 per cent of the world's children. There are limited data from prospective population incidence studies of Hib in Asia, and no data available from India. We therefore carried out th...

متن کامل

Haemophilus Influenzae B

  یکی از شایع ترین مننژیت باکتریایی در شیرخواران، هموفیلوس آنفلوانزا از نوع B (HIB) می باشد. به منظور تهیه و عرضه واکسنی جهت حفظ کودکان در مقابل این بیماری به بازار جهانی، تحقیقاتی انجام و نوعی واکسن در بخش تحقیقاتی آکسفورد مورد آزمایش و ارزیابی قرار گرفت تا در صورت تمایل والدین و کارکنان بهداشتی، تجویز همزمان آن با واکسن سه گانه و فلج اطفال عملی گردد.

متن کامل

frequency and antimicrobial susceptibility of haemophilus influenzae type b isolated from children suspected to meningitis

background: haemophilus influenzae type b (hib) has emerged as the predominant cause of meningitis in developing coun­tries wherever it has been studied using good microbiologic procedures, but data  regarding frequency, antimicrobial sus­ceptibility and preferable method for detection of haemophilus influenzae type b isolated from  iranian patients  were scarce. methods: one thousand and eight...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of microbiology

جلد ۷، شماره ۳، صفحات ۱۳۶-۱۴۳

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023